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Mix by vortexing for 15 seconds. 2. www.lucigen.com QuickExtractTM DNA Extraction Solution. 4. Transfer the tube to a heat block at 65°C and incubate for 6 minutes (15 minutes for fingernails). 5. Mix by vortexing for 15 seconds. 6. Transfer the tube to a heat block at 98°C and incubate for 2 minutes.
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The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample. The extracted DNA is suitable for PCR analyses, such as genomic, transgenic, or viral DNA screening in animals, or.
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Fast: 8 minute extraction protocol for most sample types. Simple: No centrifugation steps of spin columns used to help increase yields. Automation-friendly: Simple protocol integrated easily into automated workflows. Safe: Uses only non-toxic reagents. These products are suitable for a broad range of sample types, from swabs to hair follicles.
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Simple, rapid extraction (no purification) of DNA for PCR-based screening and genotyping type applicationsThe wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Use these reagents for isolating and purifying nucleic acids from biological material for use in further.
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Extract DNA from any sample in minutes with QuickExtract™ Solution. PCR-ready, high-quality genomic DNA for various applications.
Quick DNA Extraction of CRISPR KnockIn Cells Using Lucigen Quick DNA
This solution makes it remarkably easy to extract genomic DNA from either cell pellets or attached cells. I was able to quickly harvest gDNA from 48 clones directly from 24 well dishes just by aspirating media, washing in DPBS, and adding the QuickExtract solution directly to the wells. After harvesting, a 15 minute incubation protocol will.
QuickExtract™ DNA Extraction Solution
dilutions of the QuickExtract extract after sample processing is advised, for example 1:4, 1:8, 1:16, to determine the optimal amount of processed sample volume for your downstream application. Finally, it is important to emphasise that seeds must be cleaned efficiently if genotyping, since interpretation of results may
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Pellet the cells by centrifugation at 1,000 x g at 4°C for 5 minutes. Aspirate the supernatant. Add 100 μl of ice cold QuickExtract RNA Extraction Solution to the cells and vortex mix for 1 full minute. The RNA can be used directly in a cDNA synthesis reaction or stored frozen at -70°C.
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The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes (Fig. 1), depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of.
Quick Extract DNA Kit at Rs 75/sample DNA Kit, DNA Isolation Kit, DNA
From earth to space - QuickExtract is propelling science forward. QuickExtract™ has revolutionised genomic nucleic acid extraction. From uncovering insights on Earth to boldly venturing into new frontiers at the International Space Station - its unmatched versatility across sample types and lightning-fast results have helped propel.
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QuickExtract DNA Extraction Solution. The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
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QuickExtract DNA extraction solution • The QuickExtract™ DNA extraction solution extracts PCR-ready genomic DNA from almost any sample in just 3-8 minutes. • Many publications support the use of
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protocol has been used to extract PCR-ready genomic DNA from HeLa cells, hair follicles, tail snips, bacterial cells, fingernails, and feathers. 1. Label the appropriate number of tubes containing 0.5 ml of QuickExtract Solution. 2. Place one sample in each tube, for example: • 10. 4. counted human cervical carcinoma tissue culture (HeLa) cells.
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Apply for a Business Account Chemical Structure Search Favorites Help & Support Quick Order. Promotional price valid on web orders only. Your contract pricing may differ.. The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube.
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The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents.
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The QuickExtract solution has been used for leaf or seed material to successfully extract PCR-amplifiable DNA from: Arabidopsis, hopvine, maize, grape, rosemary, rice. perform a quick centrifugation prior to aspirating your sample. 5. The second heating step at 95-98 °C may deform some plastics. If using microtiter plates, be sure that the.